The Expertise of Bio Elpida in Eukaryotes Cell Culture
Isolation, cloning and large-scale culture for the production of proteins of interest
Bio Elpida has the facilities, equipments and expertise to handle
eukaryote cell culture projects:
- Isolation
- Cloning
- Large-scale culture…
Upstream step – Culture
For upstream steps, Bio Elpida recommends a
Single-Use approach.
The techniques used are adapted to cell suspensions as well as adhesive cells. The culture volume will depend on the project and can range from small volumes (100ml) to the manufacturing of pre-industrial clinical batches (20l or more depending of the technology).
In addition, Bio Elpida proposes to
optimise culture conditions, including medium composition.
Downstream step – Purification of API
Bio Elpida can also organise and/or participate in all downstream steps necessary to the
"production" of antibodies or proteins of interest obtained by cell culture:
- Clarification by classical techniques such as filtration and cycles of ultra-filtrations
- Purification by liquid chromatography of antibodies and of protein of interest with equipments that are compatible with development and also with the manufacturing of pre-clinical or phase I/II clinical batches
- Inactivation and sterilisation with viral inactivation by ultrafiltration/diafiltration, nanofiltration or chemical inactivation (solvent, detergent, pH…)
All these operations can be performed in the pharmaceutical zone.
